PCR Wound Sample Collection Training for Mobile Healthcare Providers

Duration: 3-4 hours (including hands-on practice and assessment)
Target Audience: Nurses, Medical Assistants, Mobile Healthcare Providers
Mode: In-person or Online with Practical Demonstration

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Course Objectives:

By the end of this course, participants will be able to:

1. Understand the principles and importance of PCR molecular testing for wound infections.
2. Identify and prepare the necessary equipment and materials for proper sample collection.
3. Perform aseptic wound sampling techniques to minimize contamination.
4. Handle and transport samples according to laboratory protocols and regulatory standards.
5. Recognize common errors and troubleshooting techniques in PCR wound sample collection.

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Course Modules:

Module 1: Introduction to PCR Testing for Wound Infections (30 minutes)

• What is PCR testing? (vs. traditional CNS culture testing)
• Why PCR for wound infections?
  • Faster and more accurate pathogen identification
  • Detection of antibiotic resistance genes
  • Quantitative data for clinical decision-making
• Key advantages in SNFs and ALFs
  • Prevents unnecessary antibiotic use
  • Reduces hospitalizations

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Module 2: Pre-Sample Collection Preparation (45 minutes)

• Understanding Wound Types & Indications for PCR Testing
  • Chronic vs. acute wounds
  • Signs of infection requiring PCR analysis
• Review of Required Materials & PPE
  • Swab collection kit
  • Transport media
  • Labels & requisition forms
  • Biohazard bags
• Infection Control & Aseptic Technique
  • Hand hygiene and glove use
  • Avoiding cross-contamination

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Module 3: Proper PCR Wound Sample Collection Technique (1 hour)

Step-by-step collection method:

1. Verify patient identity and document wound site.
2. Cleanse wound area with sterile saline (if applicable).
3. Select appropriate swab type (e.g., flocked swab).
4. Use the Levine or Z-Stroke Technique to obtain an optimal sample:
   • Levine Technique: Apply pressure and rotate swab in a 1 cm² area.
   • Z-Stroke Technique: Swab in a zigzag motion across the wound bed.
5. Place swab immediately into the transport tube.
6. Properly seal and label sample container.
7. Dispose of PPE and waste per infection control guidelines.

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Module 4: Sample Handling, Transport, and Documentation (30 minutes)

• Proper Labeling & Chain of Custody
  • Patient identifiers
  • Collection time and date
  • Provider initials
• Transporting Samples to the Laboratory
  • Refrigeration requirements
  • Courier protocols and turnaround time expectations
• Regulatory Compliance & Documentation
  • CLIA and CAP standards for sample integrity
  • Completing electronic medical records (EMR)

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Module 5: Troubleshooting and Common Errors in Sample Collection (30 minutes)

• Common Collection Mistakes
  • Dry swabs or improper transport
  • Insufficient sample quantity
  • Touching contaminated areas (e.g., skin, pus, exudate)
• How to Avoid False Negatives or Inconclusive Results
• Case Scenarios & Group Discussion

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Module 6: Hands-on Practical Session & Assessment (45 minutes)

• Live Demonstration of Proper Collection Techniques
• Guided Hands-on Practice with Feedback
• Final Competency Check & Certification Quiz

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Course Completion & Certification:

• Participants must successfully collect and handle a mock PCR wound sample.
• Pass a 10-question assessment on best practices.
• Receive a Certificate of Competency in PCR Wound Sample Collection.

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